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KMID : 0382619830030010059
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Hanyang Journal of Medicine
1983 Volume.3 No. 1 p.59 ~ p.72
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Activities and properties of amylase in cystic fluid of ovarian cancer
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Abstract
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In order to investigate whether amylase activity in ovarian cystic fluid could be used as a marker for ovarian neoplasms, activities of the enzyme were determined in cystic fluid of patients with cyst forming ovarian diseases. Also studied were properties of the amylase using the enzyme preparation which had been partially purified by centrifugation and DEAE-cellulose column chromatography.
1) Activities of amylase in ovarian cystic fluid were greatly increased in ovarian cancer, exhibiting the highest activity of the enzyme in serous cystadenocarcinoma.
2) DEAE-cellulose column chromatography of the centrifuged cystic fluid showed that two types (peak I and II) of the enzyme, active against starch, were present in ovarian cystic fluid of patients with serous cystadenocarcinoma. Studies on the enzyme revealed that the peak I and II enzymes appeared to be alpha-type of amylase.
3) The properties of peak I amylase in ovarian cystic fluid of serous cystadenocarcinoma appeared to be different from those of peak II enzyme, since two peak enzymes showed different patterns of halogen activation and substrate specificity.
4) Observations that the peak I enzyme is major enzyme in cystic fluid of serous cystadenocarcinoma and may be specific for ovarian cancer, suggest that the peak I amylase might be a marker for serous ovarian neoplasms.
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